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4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), Acyl-Coenzyme A (CoA) synthetase, Tris-(benzyltriazolylmethyl) amine (TBTA), palmitoyl CoA, Tris-carboxyethylphosphine (TCEP), lithium CoA (LiCoA), 1-chloro-2,4-dinitrobenzene (CDNB), magnesium chloride (MgCl 2 ), 1,4 - dithiothreitol (DTT), Trisma-base, Tris-HCl, reduced L-glutathione (GSH), bovine serum albumin (BSA), CuSO 4 , ATP, acetonitrile, N -ethylmaleimide (NEM), 4,6-diamidino-2-phenylindole (DAPI), Duolink in situ detection reagent orange, Duolink in situ PLA probe anti-mouse PLUS affinity purified donkey anti-mouse IgG (H+L), Duolink in situ proximity ligation assay (PLA) probe anti-rabbit MINUS affinity purified donkey anti-rabbit IgG (H+L), Duolink in situ wash buffers, were purchased from Sigma-Aldrich (Oakville, ON, Canada)

Endogenous formation of AGEs occurs continuously at low levels, but exogenous sources, including consumption of a high-sugar diet, can drastically increase this pool

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