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Description

This technique relies on the utilization of specialized antibodies that have an affinity for TMAO, enabling its identification and measurement

40 , e107821 (2021)

doi: 10.1002/rmv.2095 135 OkunoYMurataTSatoYMuramatsuHItoYWatanabeTet al

2.3 Cell culture and treatment The human normal hepatic cell line LO2 (Shanghai Institute of Biological Sciences, Chinese Academy of Science, Shanghai, China) was maintained in DMEM(BI,United States) containing 10% FBS (Gibco, United States) and 1% penicillin/streptomycin (Gibco, United States) in a 5% CO2 humidified 37C incubator