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Supplementation may cause accumulation and oxidative paradoxhigh antioxidant levels can promote pro-oxidant effects in these contexts

[DOI] [PMC free article] [PubMed] [Google Scholar] 265.Di Magno L, Coluccia A, Bufano M, Ripa S, La Regina G, Nalli M, et al

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Ellmans assay Free glutathione after plasma treatment was observed using the glutathione detection kit commercially available from Enzo Life Science (#ADI-900-160) without glutathione reductase resulting in a typical Ellmans end point detection of free thiols, which react stoichiometrically with 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB) by cleaving the disulphide bond yielding 2-nitro-5-thiobenzoate (TNB - )