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Various studies have shown that the first follicle rupture occurs about 32-38 hours after hCG injection (10) (11)

In the gourami, OAB occurred at 35 dpf in normoxia (21 kPa PO 2 ), but severe intermittent nocturnal hypoxia (14 kPa PO 2 ) delayed appearance of this developmental maker by several days (Figure 4A)

Consider staying at your current dose for an additional 2-4 weeks if you experience: Persistent nausea or vomiting lasting more than 3 days Rapid weight loss exceeding 3-4 lbs per week Severe constipation or diarrhea Signs of dehydration Significant injection site reactions The goal is to reach the maintenance dose gradually

And the big one that we think of it as is intestinal tract health