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The LC separation employed a gradient of solvent B (0.1% FA in 98% acetonitrile) from 6% to 22% over 22 min, followed by an increase to 36% over 8 min, and a final ramp to 80% over 5 min, which was maintained for 3 min

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Indeed, a markedly increased SIRT7 expression was observed when EGF signaling activity was blocked in MDA-231 breast cancer cells by erlotinib, whereas EGF stimulation accelerated SIRT7 protein turnover (Fig