1 H-NMR (400 MHz, CDCl 3 ) ppm = 7.72 (dd, J = 0.6 Hz, J = 8.2 Hz, 1H, Ar-H), 7.66 (dd, J = 0.8 Hz, J = 1.8 Hz, 1H, Ar-H), 7.63 (dd, J = 0.6 Hz, J = 1.5 Hz, 1H, Ar-H), 7.40 (dd, J = 1.5 Hz, J = 8.2 Hz, 1H, Ar-H), 7.28 (dd, J = 0.8 Hz, J = 3.5 Hz, 1H, Ar-H), 6.60 (dd, J = 1.8 Hz, J = 3.5 Hz, 1H, Ar-H), 3.11 (bs, 3 H, -CH 3 ), 3.00 (bs, 3 H, -CH 3 )
Dulbeccos Modified Eagles Medium (DMEM), fetal bovine serum (FBS), 0.25% trypsin, and penicillin-streptomycin (100) were purchased from Gibco (Grand Island, USA)
Potential therapeutic strategies It has been reported that artesunate prevents brain damage at low doses by blocking ferroptosis, and iron chelators such as DFO and DFP have demonstrated positive effects against iron-related neurodegenerative disorders
The results of ELISA and Western blot analysis showed that compared with cells without treatment, levels of IL-6 and TNF- and protein levels of Collagen I and -SMA elevated in cells treated with CSE, while levels of IL-6 and TNF- and protein levels of Collagen I and -SMA decreased in cells treated with CSE and PBS compared with cells treated with CSE plus NAC (Fig