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Abbreviations CCl 4 : Carbon tetrachloride TGF-: Transforming growth factor-beta Smad2/3: Drosophila mothers against decapentaplegic 2/3 GPME: Glycosmis pentaphylla methanolic extract ALT: Alanine aminotransferase AST: Aspartate aminotransferase ALP: Alkaline phosphatase -SMA: Alpha-smooth muscle actin IHC: Immunohistochemistry TEM: Transmission electron microscopy SEM: Scanning electron microscopy IL-10: Interleukin-10 TNF-: Tumor necrosis factor-alpha IL-6: Interleukin-6 SOD: Superoxide dismutase CAT: Catalase GSH: Glutathione MDA: Malondialdehyde GC-MS: Gas chromatography-mass spectrometry LC-MS: Liquid chromatography-mass spectrometry TGFR1: Transforming growth factor-beta receptor 1 ROS: Reactive oxygen species ECM: Extracellular matrix HSCs: Hepatic stellate cells NIST: National Institute of Standards and Technology CPCSEA: Committee for the purpose of control and supervision of experiments on animals RIPA: Radioimmunoprecipitation assay EDTA: Ethylenediaminetetraacetic acid PMSF: Phenylmethylsulfonyl fluoride TP: Total protein TBIL: Total bilirubin GGT: Gamma-glutamyl transferase TC: Total cholesterol TG: Triglycerides HDL: High-density lipoprotein LDL: Low-density lipoprotein VLDL: Very low-density lipoprotein PBS: Phosphate-buffered saline TMS: Tetramethylsilane PVDF: Polyvinylidene difluoride PBST: Phosphate-buffered saline with Tween-20 GAPDH: Glyceraldehyde-3-phosphate dehydrogenase TPSA: Topological polar surface area LGA: Lamarckian genetic algorithm ARRIVE: Animal research: reporting of in vivo experiments SDS-PAGE: Sodium dodecyl sulfate polyacrylamide gel electrophoresis HRP: Horseradish peroxidase DAB : 3,3-diaminobenzidine BSA: Bovine serum albumin NIH: National Institutes of Health References Almatroodi SA, Anwar S, Almatroudi A, Khan AA, Alrumaihi F, Alsahli MA et al (2020) Hepatoprotective effects of garlic extract against carbon tetrachloride (CCl 4 )-induced liver injury via modulation of antioxidant, anti-inflammatory activities and hepatocyte architecture

P146, P293, P582, P1101 Gassara Z

The body has a natural system to remove these reactive O2 species

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