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The tubes were vortexed and incubated in the ultrasonic bath for 10 min then centrifuged at 13,000 rpm for 20 min at 4 C

(PubMed) Mangiapane E, Pessione A, Pessione E

In addition, reported outcomes differ from trial to trial, preventing rigorous evidence synthesis, for example in forms of well-conducted meta-analyses

3,5-diCQM drives melanogenesis in a dose-dependent manner, and the molecular mechanism underlying its ability to induce pigmentation is through activation of the p38 signaling pathway, phosphorylation and activation of CREB, and a cAMP/PKA dependent signaling pathway, which in turn upregulates the transcription factor MITF, thereby activating tyrosinase activity (Kim et al., 2015)