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Then, 1 ml of glutathione Sepharose 4B (Amersham) slurry and 1 ml of tryptophan-induced culture broth were added to 40 ml of the PBS buffer in a 50 ml tube, and kept at the room temperature for 30 min, centrifuged at 1,000 g for 10 min at the room temperature, then resuspended in the PBS buffer, and centrifuged at 1,000 g for three more times