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To determine the accurate concentrations of [ 15 N 13 ]-CNCbl used for each extraction, a calibrator solution comprising unlabeled CNCbl (50-100 ng/mL) and labeled [ 15 N 13 ]-CNCbl (50-100 ng/mL) was routinely prepared and measured by LC-MS/MS

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The storage of cyanocobalamin in the body is dose-dependent

Such developments would provide researchers with an expanded toolkit for investigating the complex relationships between metabolism, aging, and disease, potentially accelerating discoveries across multiple fields of biomedical research