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It is a major cause of mortality in patients with DMD

10.1016/j.acthis.2007.12.009 85

The PCR product was subjected to restriction digestion with 10 U of the Bss T1I enzyme (Sibenzyme) at 60 C for 4 h followed by 2.5%-agarose gel electrophoresis

This balance between size and specificity is frequently cited as a design achievement of the original Monash research program and is one reason the molecule has endured as a reference compound across multiple laboratories