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Description

The raw imaging data are protected and are not available due to data privacy laws

The unmarked mutant was confirmed by PCR and RT-qPCR to confirm the loss of gene as well as transcripts of metZ gene

CRISPR/Cas9 dropout screens Genome-wide CRISPR/Cas9 dropout screens were performed in Cas9-expressing TaC12 cells using a two-vector system, as well as small-scale screens in TaC12 and BoMac cells