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For the closely eluting VVVFIKPTC(GSH)PYC(O3)R and VVVFIKPTC(O3)PYC(GSH)R peptides, the abundance was determined by summing the area of the XIC of the 3 transitions that are unique to the peptides (VVVFIKPTC(GSSG)PYC(O3)R: m/z 939.433 ++ 326.113 [y2] + ,C(GSH), 489.176 [y3] + Y, and 586.229 [y4] + P, and VVFIKPTC(O3)PYC(GSH)R: ++ 538.196 [y2] + C(O3), 746.260 [y3] + Y, and 843.312 [y4] + P) (diagnostic ions are boxed in the ion tables in Supplementary Fig

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If the test reaffirms a positive outcome, the sample undergoes gas chromatography/mass spectrometry to pinpoint the specific substance in the urine

Increased consumption of antioxidants was also reported for other members of the nonenzymatic antioxidant system, including -tocopherol and ascorbic acid (35, 86)