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The low GSH concentration necessary for CVA21 displacement may also limit a co-elution of potentially bound host cell GST enzymes present in cell culture harvests, which may require as high as 10 mM GSH for elution (Figure 4A) and elution NaCl gradient (Supplementary Figure S6C) studies where CVA21 particles eluted at lower ionic strengths with increasing pH

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However, the results should be interpreted with caution due to significant heterogeneity in most comparisons

A noticeable finding in several of these trials was that patients failed to achieve the higher hemoglobin target despite the use of very high doses of ESA