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Specific inhibition of HK2 with 3-bromopyruvate reduces joint swelling and histological damage in SKG mice, a RA model, by suppressing glycolysis-dependent dendritic cell activation and Th17/Treg imbalance

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10.1016/j.exger.2020.111062

Cells were first stained with 50 M DCFDA or 10 M mBcl, as described above, followed by a washing step with PBS and a surface staining of 30 min on ice with combinations of the following monoclonal antibodies: PE-conjugated anti-CD10 (HI10), PerCP-conjugated anti-CD3 (SK7), APC-conjugated anti-CD19 (SJ25C1), PE-conjugated anti-CD5 (L17F12), PE-conjugated anti-CD25 (PC61), all from BD Biosciences