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ADPWH_ lux was diluted in 37C LB (1:20) and grown for 3 h at 200 rpm to an OD 600 of 0.4

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An alternative approach that has been explored experimentally in the porcine ischemic injury model to limit formation of LA inhibitory fragments while maintaining the ability of LA to induce recovery of barrier function involves addition of small doses of LA over time and suggest that 0.1 M LA applied every 45 min is effective (49)

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