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Consequently, we delve into the molecular mechanisms through which 16-OHE promotes PH, encompassing induction of oxidative stress, enhancement of proliferation and migration of pulmonary arterial smooth muscle cells (PASMCs) and pulmonary arterial endothelial cells (PAECs), promotion of metabolic abnormalities, induction of endothelial mesenchymal transition (EndoMT), and fostering inflammation (Fig