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Cellular extracts were added in a final volume of 1.0 ml, to 150 mM Tris HCl buffer, pH 8.2, containing 25 mM L-glutamate, 25 mM L--aminobutyrate, 10 mM ATP, 10 mM phosphoenolpyruvate, 30 mM MgCl 2 , 100 mM KCl, 0.2 mM EDTA, 0.42 mM NADH, 7 IU pyruvate kinase, 12 IU lactate dehydrogenase, and the oxidation of NADH was monitored at 340 nm

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Finally, the relative contributions of direct neuronal regulation versus indirect effects via immune cells (e.g., macrophages) to the observed anti-inflammatory effects remain undetermined

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