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cerevisiae invertase, rabbit protein phosphatase-1 (PP1) and T4 DNA ligase have been demonstrated using the IMPACT system ( Although the original IMPACT system only provided N-terminal intein cleavage (where the target protein is cloned upstream of the intein), the subsequent IMPACT-CN system allowed for either N or C-terminal cleavage, with an extra dialysis step required for C-terminal cleavage (Figure 3)