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doi: 10.3389/fnut.2025.1650328 Received 19 June 2025 Accepted 20 August 2025 Published 03 September 2025 Volume 12 - 2025 Edited by Karolina Wojtunik-Kulesza, Medical University of Lublin, Poland Reviewed by Sara Luz Morales-Lzaro, National Autonomous University of Mexico, Mexico Rogrio Saad Vaz, Federal University of Paran, Brazil Anna Oniszczuk, Doctoral School of Medical University of Lublin, Poland Updates Copyright 2025 Ye, Sun, He, Ai, Jin and Abd El-Aty

Wyrzykowska, A

Further research by Shi et al

In vitro embryo culture At 18 h post insemination (hpi), the putative zygotes were washed by repeated pipetting and transferred to 25 L drops (approximately one embryo per L) of culture medium (SOF supplemented with 3 mg/mL bovine serum albumin), covered with mineral oil and cultured until Day 8 post insemination (dpi) at 38.5 C in a humidified atmosphere with 5% CO 2 , 5% O 2 and 90% N 2 in air