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Another recurring limitation emerges on antioxidant-based interventions in ASD, constrained by a fundamental methodological gap: most clinical studies report behavioral or functional improvements without directly measuring oxidative-stress biomarkers

In one randomized, placebo-controlled trial in 60 women with gestational diabetes, supplementation with omega-3 fatty acids and vitamin E reduced the risk of neonatal hyperbilirubinemia yet had no effect on the rate of cesarean section, need for insulin therapy, maternal hospitalization, newborns' hospitalization, gestational age, birth size, and Apgar score (122)

Carnitine supplementation leads to elevated plasma levels of TMAO [64]

Thus, in a randomized clinical trial conducted in selenium-sufficient GD patients treated with methimazole, no beneficial effect of Se on the short-term control of thyroid hyperfunction was observed (82)