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Association of long-term proton pump inhibitor use with nutrient deficiencies: a retrospective cross-sectional study

J., Sevanian, A., Muakkassah-Kelly, S

To improve the sensitivity and accuracy of the assay, we first cultured human MCF-7 cells in heavy media (containing K8 [ 13 C 6 , 15 N 2 ] heavy isotope-labeled l-lysine) and enriched cellular histone peptides bearing the PTMs of interest using pan anti-K l-la , pan anti-K d-la and pan anti-K ce antibodies