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At this stage of purification GST-X fusion should be clean and depending on the nature of furhter experiments that we want to perform, we can either elute the fusion with glutathione (excess of the glutathione will compete and displace GST-X protein from the beads) or cleave the GST tag and release protein X (see cartoon below)

Glutathione then regenerates vitamin C, and NADPH restores glutathione from its oxidized form (GSSG)

Use of SSRIs did not influence brain levels of GSH (ACC n = 12/30: p = 0.275

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